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Advanced Course
CURRENT AND FUTURE DIAGNOSTIC METHODOLOGIES
IN ANIMAL HEALTH
Zaragoza (Spain), 14-18 January 2013
Objective
of the course
Organization
Admission
Registration
Scholarships
Insurance
Teaching organization
Programme
Guest lecturers
The livestock industry
contributes largely to the economic and social welfare of the rural populations
in the Mediterranean countries. A number of animal diseases continue to
represent a serious constraint to the development of this sector as well as an
important barrier for the international animal trade. Accordingly, the proper
diagnosis of these diseases is of paramount importance.
An effective response to
disease outbreaks requires a comprehensive diagnostic plan that addresses each
phase of the outbreak: surveillance, response and control. The diagnostic
laboratory thus plays an essential role in the surveillance system. A broad
spectrum of sensitive and specific diagnostic assays is crucial in providing
timely and accurate diagnosis for immediate response for disease control.
This course is dedicated to
qualified staff in veterinary diagnostic laboratories to provide them with the
next generation and current methodologies in animal disease diagnosis so as to
enhance the control/eradication strategies and achieve sustainable impact on
the development of the livestock industry.
Upon completion of the
course, the participants will have gained expertise on the following areas:
-
Understanding the relevance
of laboratory management, quality assurance and compliance with international
standards in providing laboratory results with high level of accuracy and
confidence.
-
Acquire information about
the role of international organizations and the benefits of networking with the
reference laboratories in diagnostics.
-
Overview of classical and
new diagnostic technologies and their potential use in the Animal Health area.
-
Application of the
diagnostic methods on selected case studies of major diseases in the
Mediterranean region.
The course is jointly organized
by the International Centre for Advanced Mediterranean Agronomic Studies
(CIHEAM), through the Mediterranean
Agronomic Institute of Zaragoza
(IAMZ), the Food and Agriculture Organization of the United Nations (FAO),
through the Animal Production and Health Division, and the World
Organisation for Animal Health (OIE), and will take place at the Mediterranean Agronomic Institute of Zaragoza.
The course will be given by well-qualified lecturers from international
organizations and from research centres, universities and government
departments in different countries.
The course will be held over a period of one week, from 14 to 18 January
The course caters for 25
participants with a university degree
and is aimed at qualified staff in public and private veterinary diagnostic
laboratories. The course would also be opened to researchers involved in animal
health diagnosis.
Given the diverse nationalities
of the lecturers, knowledge of English and French will be valued in the
selection of candidates, since together with Spanish,
they will be the working languages of the course. However, if necessary, the
Organization will provide simultaneous translation of the lectures.
Application forms may be obtained from:
Instituto
Agronómico Mediterráneo de Zaragoza
Avenida de Montañana
1005, 50059 Zaragoza (Spain)
Tel.: +34
976 716000 - Fax: +34 976 716001
e-mail: iamz@iamz.ciheam.org
Web:
www.iamz.ciheam.org
Candidates should send the completed application form to the above
address, accompanied by a detailed curriculum vitae, stating degree,
diplomas, experience, professional activities, language knowledge and reasons
for applying to the course. Copies of certificates should be enclosed with the
application.
The deadline for the submission of applications is 5 November 2012.
Applications from those candidates who cannot present their complete
records when applying, or those requiring authorization to attend the course,
may be accepted provisionally.
Registration fees for the course amount to 450 euro. This sum covers
tuition fees only.
Candidates from CIHEAM member countries (Albania, Algeria, Egypt,
France, Greece, Italy, Lebanon, Malta, Morocco, Portugal, Spain, Tunisia and
Turkey) may apply for scholarships covering registration fees, and for
scholarships covering the cost of travel and full board accommodation in the
Hall of Residence on the Aula Dei Campus.
Candidates from other countries who require financial support should
apply directly to other national or international institutions.
It is compulsory for participants to have medical insurance valid for
Spain. Proof of insurance cover must be given at the beginning of the course.
Those who so wish may participate in a collective insurance policy taken out by
the Organization, upon payment of the stipulated sum.
The course requires personal
work and interaction among participants and with lecturers. The international
characteristics of the course favour the exchange of experiences and points of
view.
The programme has an applied approach, combining
specialized lectures with case studies and debates. Participants will be
invited to prepare before the beginning of the course a brief report on their
own professional experiences regarding
diagnosis procedures in livestock health management. These reports will be
shared with lecturers and participants.
1.
Diagnostic laboratories and international standards (6
hours)
1.1. The diagnostic laboratory as a part of animal health
surveillance systems
1.2. The role of international organizations in disease
diagnosis
1.2.1.
The OIE Manual of
Diagnostic tests as a guide for diagnosing the main animal diseases. Importance
of standardisation and validation of diagnostic techniques for pathogens of
relevance for international trade
1.2.2.
The role of FAO and OIE
reference laboratories network. Conditions to be fulfilled to become a
reference lab
1.2.3.
Networking initiatives in
the Mediterranean region: REMESA
1.3. Management of laboratory system, biosafety/biosecurity
and quality assurance
1.4. Round table discussion: Diagnostic challenges in the
Mediterranean region
2.
Disease surveillance (3 hours)
2.1. Sampling design
2.2. Sample collection, preservation and shipping to
national and international reference labs
2.3. Use of different matrices for the diagnosis of animal
diseases
2.4. Performance of diagnostic tests
2.5. Diagnostic strategy used in different stages of
disease control programmes
3.
Diagnostic methodologies (13 hours)
3.1. Isolation of pathogens
3.1.1.
Viral diseases
3.1.2.
Bacterial diseases
3.2. Immunoassays and their applications
3.2.1.
Brief review of classical
methodologies
3.2.2.
New methods
3.2.2.1.
Luminex
3.2.2.2.
Penside test
3.2.2.3.
Fluorescence polarization
assay
3.2.3.
Tests detecting cellular
immune responses
3.3. Genomic techniques
3.3.1.
Pros and cons of genomic
analysis. Internal and external controls
3.3.2.
Extraction methodologies
for genomic diagnosis according to the type of sample and pathogen
3.3.3.
Single and multiplex
conventional and real time PCR
3.3.4.
LAMP
3.3.5.
Other molecular
characterization techniques (RFLP, RAPD, microsatelites,
PGFP)
3.3.6.
Sequence analysis
3.3.6.1.
Ultrasequencing
3.3.6.2.
Spiral sequencing
3.3.6.3.
Real time full genomic
sequencing
3.3.7.
Microarrays
3.4. Proteomics
3.4.1. Separation techniques: conventional 2DE, 2DE-DIGE, LC
3.4.2.
Identification techniques:
MALDI-TOF-MS and MS/MS
3.4.3.
Labelling and
quantification techniques
3.4.4.
Experimental designs, MIAPEs (minimum information about a proteomic experiment)
and validation
3.4.5.
Present and future
applications in animal health and disease diagnosis
3.5. Open discussion: Implementing diagnostic methods in
participants’ countries
4.
Diagnosis case studies of major diseases in the
Mediterranean region (6 hours)
4.1. Bacterial diseases
4.1.1.
Brucellosis
4.1.2.
Tuberculosis
4.2. Viral disease
4.2.1.
Foot and mouth disease
4.2.2.
Vector borne diseases: blue
tongue and Schmallenberg disease
4.3. Prion diseases: Scrapie
4.4. Parasitic diseases: Toxoplasmosis
5.
Round table discussion: Future trends in disease
diagnosis in the Mediterranean region (1 hour)
C. ACÍN, Univ. Zaragoza (Spain)
G. ADURIZ, Neiker, Derio (Spain)
G. ÁLVAREZ, Saluvet, UCM, Madrid
(Spain)
J.M. BLASCO, CITA-GA, Zaragoza (Spain)
E.COLLANTES, Saluvet, UCM, Madrid
(Spain)
F. CONRATHS, FLI, Wusterhausen
(Germany)
F. GARCÍA MARÍN, Univ. León (Spain)
F. GARCÍA PEÑA, Laboratorio Central de Veterinaria de
Algete, Madrid (Spain)
C. GIL, UCM, Madrid (Spain)
S. METWALLY, FAO, Roma (Italy)
P. MUÑOZ, CITA-GA, Zaragoza (Spain)
L.M.
ORTEGA, Saluvet, UCM, Madrid (Spain)
A. PETRINI, OIE, Tunis (Tunisia)